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APPLICATION NOTE

Scanning Viral Genomes with 300mer Oligonucleotides

Researchers use long custom oligos to screen viral genome stability with an NGS workflow.


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Complex RNA structures facilitate viral replication and block antiviral host factors. To identify the sequences giving rise to these structures and determine their function, researchers recently developed a next generation sequencing (NGS)-based screening workflow called Fate-seq. This technique utilizes custom 300mer oligonucleotides to scan across genomes in a high-throughput manner. Using Fate-seq, researchers screened genomic sequences long enough to identify these complex secondary structures in the SARS-CoV and SARS-CoV-2 genome.

Download this application note from Twist Bioscience to learn about

  • Custom oligonucleotides and the Fate-seq workflow
  • Identifying high-dimensional RNA structures with long oligos and NGS
  • How SARS viruses may evade host immune responses